Highlights:
1. Plant microspores can be switched from their normal pollen developmental pathway towards an embryogenic route.
2. The application of a stress treatment is necessary for the reprogramming of microspores leading to an intermediate stage of dedifferentiation and cell totipotency.
3. Under appropriate culture conditions, the microspore starts proliferation and a cell fate developmental program to embryogenesis.
4. Our aim is to gain a better understanding of the developmental regulation of the microspore embryogenesis process, to identify target genes and to perform an integrate analysis that allow us the improvement of tools for double haploid production programs. Previous experience on gene expression analysis, Real time PCR and in situ hybridization will be valued.
5. Last date: 2 December 2009
ESTACION EXPERIMENTAL AULA DEI (EEAD)
CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS (CSIC)
Av de Montañana 1005, 50059 ZARAGOZA, SPAIN
Contact:
María-Pilar Vallés, Ana-María Castillo
valles@eead.csic.es - amcast@eead.csic.es
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